Figure 3.

The effect of satraplatin-induced cell cycle perturbation at the G₂/M phase, apoptosis and cyclin-B1 expression in CRC cells at 48 h. (A) Table showing an accumulation of cells at each phase of the cell cycle following satraplatin treatment at lower and higher concentrations. (B) Cyclin B1 protein expression levels following treatment with 5 and 10 μmol/L satraplatin in CRC cells harvested at 48 h. The results shown are representative western blots of at least two independent experiments. Equal loading was confirmed by β-actin detection. (C) The effects of various satraplatin concentrations on DNA synthesis and cell cycle progression in CRC cells. Typical cytograms are presented that represent the overlaps of the percentage of cells in each phase (G₀/G₁, S, and G₂/M) compared with untreated (control) cells (red background: untreated control cells; white background: treated cells) as determined by flow cytometry. This analysis indicates that the cells were substantially past the initial entry to G₂ after DNA damage and were subsequently arrested.