Figure 2.

In vivo study of CrTX in A549 tumor xenograft model. Five million A549 cells were implanted into female nude mice subcutaneously. When the tumor reached about 5–6 mm in diameter, the tumor-bearing mice were randomized into 2 groups with 8 mice in each group and treated with vehicle control and CrTX (10 μg/kg, ip, twice a week). (A) After 4 weeks treatment, the mice were sacrificed, and the tumor xenografts were removed, sectioned and weighted. (B) Tumor xenografts were observed under microscope and microvascular density (MVD) was counted by Weidner methods. (C) In the meanwhile, 0.5 mL of blood was harvested from each mouse and 0.2 mL of serum was collected after centrifuging for 10 min at 3000 r/min. VEGF level was thus analyzed in each serum sample by ELISA. Data from each mouse sample were analyzed and shown in bar graph with statistic analysis as mean±SD. ^bP<0.05.