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. 2011 Oct 3;32(11):1411–1418. doi: 10.1038/aps.2011.121

Figure 3.

Figure 3

Identification of pBTM116-A2IC yeast expression plasmids. (A) A2IC PCR product. (B) Digested A2IC with BamH I. (C) Digested and dephosphorylated pBTM116 plasmid with BamH I. All digestions were separated by horizontal 1% agarose gel electrophoresis in 0.5×TBE buffer containing ethidium bromide (10 μg/mL). (D) Cloning of PCR products (A2IC fragment). Transformants (pBTM116-A2IC) were amplified with the primers Anx2-F and Bam-Anx2-R. (E) Positive constructs (1–7) (pBTM116-A2IC): extracted plasmid from the transformants was digested with BamH I. (F) Sense constructs (1–6) (pBTM116-A2IC): extracted plasmid from the transformants was digested with EcoR I.