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. 2010 May 5;31(5):629–637. doi: 10.1038/aps.2010.39

Figure 2.

Figure 2

MK inhibits the differentiation of mESCs in long-term cultures. (A) ALP and Nanog ICC staining of mESCs cultured with increasing concentrations of MK (from left to right: 0, 10, 20, and 40 ng/mL) at passage 7, scale bars=20 μm. (B) Expression of Oct4 and Nanog in mESCs at 0–40 ng/mL MK at passage 7, with β–actin as an internal control. (C) ALP and Nanog ICC staining of mESCs with 20 ng/mL MK at passage 1–15 (from left to right: P1, P5, P10, and P15), scale bars=20 μm, and (D) the expression of pluripotency markers Oct4 and Nanog.