Figure 6.

Effects of miR-221 and anti-miR-221 on cell proliferation and apoptosis in CRC cells. (A) The status of cell proliferation was determined by MTT assay. CPIR on the presence of miR-221 or anti-miR-221 was compared with those of the controls. n=6. Mean±SD. ^cP<0.01 vs control group. (B) Effects of miR-221 and anti-miR-221 on cell-cycle in Caco2 cells. The percentages of G₀/G₁ phase, S phase and apoptotic rate were measured by computing the ratio of the number of corresponding cells and that of total cells. n=3. Mean±SD. ^cP<0.01 vs control group. (C, D) Cell apoptosis analysis of transfected cells by flow cytometer. Note: C, NC group; D, anti-miR-221 group. The right-down quadrant (FITC⁺/PI^-) was shown as apoptotic cells.