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. 2011 Jan 31;32(3):375–384. doi: 10.1038/aps.2010.206

Figure 7.

Figure 7

Ectopic expression of miR-221 affects the growth of CRC cells by targeting CDKN1C/p57. Caco2 cells were pretreated with or without anti-p57-siRNA (80 nmol/L) for 24 h prior to the addition of pre-miR-221 (50 nmol/L) or anti-miR-221 (50 nmol/L). Semi-quantitative RT-PCR and Western-blot analysis showing that both CDKN1C/p57 mRNA (A) and protein (B) reduced markedly after transfection with anti-p57-siRNA. Equal loading was confirmed by using GAPDH or β-actin. Note: lane 1, negative control; lane 2, Caco2 cells transfected with control siRNA; lane 3, Caco2 cells transfected with anti-p57-siRNA. (C) The status of cell proliferation was determined by MTT assay. The suppression of Caco2 cell proliferation by anti-miR-221 was largely, but not completely, abrogated by anti-p57-siRNA (CPIR from 61.4% to 41.2%). n=3. Mean±SD. cP<0.01 vs negative control group. fP<0.01 vs sole anti-miR-221 group.