Figure 3.

Transfection of miR-128 inhibitor significantly suppressed Rh2-induced miR-128 over-expression in U281 cells (A), T98MG cells (B), and A172 cells (C). The effects of miR-128 inhibitor transfection on Rh2-induced miR-128 overexpression in human U251, T98MG and A172 glioma cells were determined using qRT-PCR. The cells were transfected with 50 nmol/L miR-128 inhibitor or inhibitor control for 48 h, and then with Rh2 (12 μg/mL) for 24 h. The experiment was performed three times, each in triplicate. Data were expressed as means±SEM. ^bP<0.05 compared with control. ^eP<0.05 compared with Rh2 alone or miR-128 inhibitor control+Rh2.