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Rh2 inhibited viability of human glioma U251 cells determined using a CCK-8 assay. (A) Dose-dependent response. U251 cells were treated with Rh2 (3, 6, 12 and 25 μg/mL) for 48 h. (B) Time-dependent response. U251 cells were treated with Rh2 (12 μg/mL) for 12, 24, 48 and 72 h. The results represented three independent experiments. Data were expressed as means±SEM. bP<0.05 compared with control.
