Figure 5.

Transfection of miR-128 inhibitor prevented Rh2-induced cytotoxicity in glioma U251 cells. U251 cells were transfected with 50 nmol/L miR-128 inhibitor to knockdown miR-128. A miR-128 inhibitor control was used as negative control. At 48 h after transfection, the cells were treated with 12 μg/mL Rh2 for 24 or 48 h. Cell proliferation was determined using CCK-8 assay. ^bP<0.05 compared with Rh2+miR-128 inhibitor. The experiment was performed three times, each in triplicate.