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. 2011 Mar 4;32(3):345–353. doi: 10.1038/aps.2010.220

Figure 6.

Figure 6

Transfection of miR-128 inhibitor inhibited Rh2-induced apoptosis in U251 cells. The cells were transfected with 50 nmol/L miR-128 inhibitor or inhibitor control for 48 h and then with Rh2 (12 μg/mL) for 48 h. Apoptosis was evaluated by annexin V and propidium iodide staining. The LR and UR quadrants indicated the percentage of early and late apoptotic cells, respectively, in control (A), Rh2 alone (B), Rh2+miR-128 inhibitor control (C) and Rh2+miR-128 inhibitor group (D). The experiment was performed three times, each in triplicate. Each point represented the means±SEM. There were significant differences between Rh2 alone or Rh2+miR-128 inhibitor control and control group, as well as between Rh2 alone and Rh2+miR-128 inhibitor group (P<0.05).