Figure 1.

The effects of PIMT siRNA and AdOX treatments on Aβ production. (A and B) HEK293 APPsw cells were treated with or without PIMT siRNA and AdOX. The levels of Aβ₄₀ peptide were analyzed using ELISA. (C) HEK293 APPsw cells were transiently transfected with PIMT siRNA at a concentration of 30 nmol/L. RT-PCR of RNA isolated at 48 h shows PIMT mRNA levels were reduced by PIMT siRNA. As a loading control, GAPDH mRNA levels were examined in HEK293 APPsw cells. Expression levels of PIMT protein were analyzed using a Western blot with rabbit anti-PIMT antiserum. As a loading control, levels of β-actin were also determined. (D) HEK293 APPsw cells and SH-SY5Y cells were treated with AdOX. Cell viability was determined using an MTT assay. Results are expressed as the mean±SD for three independent experiments. Mean±SD. ^bP<0.05, ^cP<0.01 compared to untreated or control group.