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. 2013 Feb 18;34(4):480–486. doi: 10.1038/aps.2012.166

Figure 2.

Figure 2

IGF1R expression is inhibited in AGE-promoted calcification in HASMCs. (A) Western blot analysis identified the overexpression of IGF1R by a lentiviral vector in HASMCs. (B) HASMCs stably transfected with IGF1R were treated with 2 mmol/L Pi in the presence or absence of 100 μg/mL AGE-BSA for 7 d, and extracellular calcium measurements were performed. (C) Western blot analysis confirmed IGF1R knockdown via the lentiviral vector in HASMCs. (D) HASMCs stably transfected with IGF1R siRNA were treated with 2 mmol/L Pi in the presence or absence of 100 μg/mL AGE-BSA for 7 d, and extracellular calcium measurements were performed. The data are presented as the mean±SD of three independent experiments performed in duplicate. bP<0.05.