The results of cell viability-MTT assay. (A) The toxic effect of SYA (0–200 nmol/L) was measured in neonatal rat cardiomyocytes after 4 h incubation by MTT. (B) Different concentrations of SYA (40, 60, and 80 nmol/L) were pretreated 24 h prior to A/R. The cells were divided into six groups: control, model (A/R), NAC (200 μmol/L+A/R), SYA-40 (40 nmol/L+A/R), SYA-60 (60 nmol/L+A/R), SYA-80 (80 nmol/L+A/R). Data were presented as mean±SD of three experiments. cP<0.01 as compared with control. fP<0.01 as compared with the model group.