Figure 1.

Strategy for constructing recombinant vaccine phage T4-mFlt4 from integration plasmid pRH-mFlt4 and phage expression vector T4-Z1. The pRH-derived plasmid contains a partially deleted T4 phage lysozyme gene e^R and endonuclease gene denV^L, which allow homologous recombination at both ends of the SOC gene with the T4-Z phage vector, on which another part of gene e⁻ and denV were deleted. The recombination produced an entire gene e⁻ and incorporated any target foreign gene X into the phage T4 genome as an SOC fusion gene.