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. 2009 Apr 6;30(5):605–616. doi: 10.1038/aps.2009.8

Figure 2.

Figure 2

Dau inhibited IGF-I-induced HIF-1α protein accumulation in MCF-7 cells but had no effect on HIF-1α mRNA levels. (A and C) Serum-starved MCF-7 cells were pretreated with different concentrations of Dau for 1 h, followed by incubation with IGF-I (40 ng/mL) for 6 h. HIF-1α protein and mRNA levels were determined by Western blotting (A) and real-time PCR (C), respectively. (B) Cell viability was analyzed using the MTT assay. (D) MCF-7 cells were pretreated with different concentrations of Dau for 1 h, followed by exposure to hypoxia for 6 h. Western blotting was performed to determine HIF-1α protein levels. Data represent mean±SD of three independent experiments.