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. 2009 Apr 6;30(5):605–616. doi: 10.1038/aps.2009.8

Figure 3.

Figure 3

Dau inhibited VEGF expression and transcriptional activation in MCF-7 cells in response to IGF-I. (A and B) Serum-starved MCF-7 cells were pretreated for 1 h with different concentrations of Dau, followed by exposure to IGF-I (40 ng/mL) for 6 h. VEGF protein production in the conditioned medium was determined by ELISA (A). VEGF mRNA expression was analyzed by real-time PCR (B). (C) Following transient transfection with VEGF promoter reporter plasmids, MCF-7 cells were pretreated with various concentrations of Dau for 1 h and then exposed to IGF-I for 6 h. Luciferase activities were analyzed using the Dual-Luciferase Assay Kit. Data represent mean±SD of three independent experiments. cP<0.01 vs drug-untreated control (IGF-I only, lane 2). Lane 2 was compared with lane 1, P<0.01.