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. 2010 Feb 5;31(2):184–190. doi: 10.1038/aps.2009.189

Figure 1.

Figure 1

Representative patterns of electrophoresis of allele specific primer-polymerase chain reaction (ASP-PCR) products with different primers for determination of 808G/T genotypes of the SLC22A2 gene. When the PCR products were observed in the lanes of 808G primers and no PCR products were observed in lanes for 808T primers (359 bp and 360 bp, respectively) , the strain was diagnosed as wild-type (wt) (A). When the PCR products were observed in the lanes of 808G and 808T primers (359 bp and 360 bp, respectively), the strain was considered as heterozygote (A). When the PCR products were observed in the lanes of 808T and no PCR products were observed in lanes for 808G primers, the strain was diagnosed as mutant homozygotes. (B) Applying the clear genotype of 8 samples as control for each test.