Figure 4.

The inhibitory effect of 10 μmol/L AA on NF-κB activity in HK-2 cells. HK-2 cells were transfected with NF-κB luciferase gene and were subsequently incubated with 10 μmol/L AA for 24 h. Experiments were done in triplicate and all results of luciferase assay expressed as relative luciferase units (RLU). Relative luciferase activity was calculated by dividing RLU of non-transfected cells, empty vector (pcDNA 3.1)-transfected cells, and AA-treated NF-κB-transfected cells by the RLU of untreated NF-κB-transfected cells. Error bars represent mean±SD of relative luciferase activity. ^cP<0.01 vs untreated NF-κB-transfected cells.