Figure 2.

Simvastatin impacts macrophage adhesion and infiltration in ApoE^−/− mice. Scanning electron microscope analysis of macrophage adhesion and subendothelial migration in the aortic arch (A–D). At higher magnification (insert), many pseudopodia were apparent around the macrophages (C). The red arrows indicate macrophages adhering to endothelial cells, and the white arrows indicate subendothelial macrophages. Macrophage accumulation in atherosclerotic lesions was examined by immunohistochemical staining of F4/80 (E–G). The special extent marked by white lines shown in F and G was used to detect the F4/80 positive area, and the relative number of macrophages in the lesion was quantified (H); n=3 per group. All scale bars=100 μm. ApoE^−/−/S: ApoE^−/− mice treated with simvastatin. ^cP<0.01 vs wild-type. ^eP<0.05 vs ApoE^−/−.