Figure 4.

Effects of HBSP on cell cycle alterations of B[alpha]P-treated Huh-7 hepatoma cells. 1 × 10⁴ of Huh-7/HBSP/B[alpha]P or Huh-7/GFP/B[alpha]P cells in 6 well plates were serum-starved for 24 h, the cells were harvested at the time of 2, 4, and 6 days after serum starvation. Cells were fixed with ice-cold 70% ethanol (prechilled at -20°C), washed with PBS, incubated with 0.05 mg/ml PI and 1 μg/ml RNase A at 37°C for 30 min in the dark. The DNA content of 10,000 cells was detected by flow cytometry and analyzed by CXP 2.2. software. The percentage of each phase of the cell cycle was determined. The results were one representative data from three independent experiments.