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Effects of H5N1-NS1 variants on p53 transcriptional activity. Saos-2 cells were transfected with p21-luc (a) or MDM-luc (b), pRL-CMV and NS1 plasmids with or without pcDNA3-p53. At 36 h post-transfection, cells were harvested and analyzed for luciferase expression. Relative activities are shown as means±SD from three independent experiments. NS1: non-structural protein 1.
