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. 2011 Aug 8;9(1):45–53. doi: 10.1038/cmi.2011.23

Figure 2.

Figure 2

Resting natural killer (NK) cells potently induce dendritic cell (DC) maturation. (a) Cultured immature DCs (iDCs) were incubated alone (iDC); with NK cells and IL-2 (NK+IL-2); with NK cells, IL-2 and poly(I:C) (NK+IL-2+I:C); with NK cells, IL-2, poly(I:C) and interferon (IFN)-α (NK+IL-2+I:C+α). The expression of CD86, CD83, CCR7 and CD38 on DCs that were induced by resting NK cells (shaded histogram) was significantly higher than that on DCs induced by activated NK cells (open histogram). Open histograms (dotted line) show the background staining with the isotype control. The data shown were obtained in a single experiment and are representative of three independent experiments. (b) IL-12p70 production after subsequent stimulation of DCs with CD40L-transfected J558 cells. Resting NK cells cultured DCs to produce significantly higher levels of IL-12p70 than did activated NK cells (*P<0.05). (c) IFN-γ production in NK cells. Purified NK cells were cultured with DCs alone or with cytokines and/or DCs. IFN-γ production during cultivation was measured. The data are shown as the mean (pg/ml)±s.d. of values from triplicate cultures obtained in a single experiment and are representative of four independent experiments.