Figure 6.

Specific killing activity of cytotoxic T lymphocytes (CTLs) was measured by ⁵¹Cr release assays. The CTLs induced by ARH77-loaded DCs were incubated with ⁵¹Cr-labeled target cells at an effector-to-target ratio (E/T) of 20∶1 or 10∶1. The amount of ⁵¹Cr released from the lysed target cells was measured. The CTLs stimulated by resting natural killer (NK) cell-stimulated DCs exhibited higher specific killing activity against ARH77 cells than those stimulated by activated NK cell-stimulated DCs (*P<0.05). Anti-major histocompatibility complex (MHC) I-blocking antibody was used to confirm the MHC class I dependence of target cell recognition. Data are shown as the means of values from triplicate cultures from a single experiment and are representative of three similar independent experiments.