Skip to main content
. 2011 Oct 10;9(1):75–85. doi: 10.1038/cmi.2011.37

Figure 6.

Figure 6

Insulin injections effectively reversed the changes observed in CD4+CD25+Foxp3+ Tregs obtained from mice with diabetes for 4 months. The mean percentage of CD4+CD25+Foxp3+ Tregs out of total CD4+ T cells in PBLs (a), the spleen (b), pLNs (c) and mLNs (d) from control and diabetic mice. (e) Representative FACS staining for the indicated molecules on gated CD4+CD25+ Tregs from control mice, diabetic mice and diabetic mice treated with insulin. (f) The percentage of indicated molecules among CD4+CD25+ Tregs in the spleen. Data are shown as mean±s.d. (n=6), for one representative out of two independent experiments. *P<0.05, **P<0.01 and ***P<0.001, comparison was among the indicated groups. CTLA-4, cytotoxic T lymphocyte-associated antigen 4; FACS, fluorescence-activated cell sorting; GITR, glucocorticoid-induced tumor-necrosis factor receptor; mLN, mesenteric lymph node; PBL, peripheral blood lymphocyte; pLN, peripheral lymph node; Treg, T regulatory cell.