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. 2011 Oct 31;9(1):54–61. doi: 10.1038/cmi.2011.40

Figure 8.

Figure 8

An anti-EGFR antibody binds to the EGFR on HeLa cells in a dose-dependent manner. An anti-EGFR antibody was labeled with FITC and then incubated with HeLa cells. (a) The percentage of HeLa cells bound by anti-EGFR was detected by flow cytometry. Negative control without any anti-EGFR antibody. The concentrations of the anti-EGFR antibody were 0.1 and 1 µg/ml. (b) HeLa cells were incubated with different concentrations of purified and FITC-labeled cancerous IgG. The percentage of PBMCs bound by cancerous Ig was determined by flow cytometry. The concentrations of cancerous IgG were 0.1, 1, 10 and 100 µg/ml.