Figure 6.
α-TOS decreased the transient activation of NFκB caused by hrTRAIL. MCF7 (A) and MDA-MB-453 (B) cells were treated with α-TOS (50 μmol/L) and/or hrTRAIL (30 ng/mL) or TNF-α (100 U/mL), and washed with PBS. Nuclear proteins were prepared and probed for NFκB activation using the TransAMTM NFκB p65 kit. The level of NFκB activation (p65 binding to its cognate DNA sequence) is expressed as absorbance at 450 nm.