Figure 4.

Tanshinone IIA induces Akt phosphorylation via PI3K. (A) Neonatal cardiomyocytes were incubated with 3 μmol/L tanshinone IIA for the indicated times. (B) Neonatal cardiomyocytes were incubated with wortmannin (Wort; 100 nmol/L) or LY294002 (LY; 10 nmol/L) for 30 min followed by incubation with 3 μmol/L tanshinone IIA for 15 min. (C) Neonatal cardiomyocytes were incubated with tanshinone IIA (3 μmol/L) for 30 min prior to the addition of Ang II (0.1 μmol/L) for 1 or 24 h. Western blot analyses were performed using site- and phospho-specific Akt antibodies against Ser473 (p-Akt, upper blot) or total Akt (lower blot). The results were shown as means±SEM (n=4), expressed as percentage changes in phosphorylation over that in control cells. ^bP<0.05 vs control (Ctrl). ^eP<0.05 vs tanshinone IIA.