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Effects of casticin on the mRNA expression of ERα and ERβ in pituitary cells stimulated with E2. Pituitary cells were stimulated with E2 (0.01 μmol/L) for 4 h and then treated with casticin for 48 h. E2+CAS0.1, E2+CAS1, and E2+CAS10 represent the expression of ERα or ERβ mRNA, expressed as the percentage of expression by the controls, in pituitary cells treated with casticin at doses of 0.1, 1, and 10 μmol/L, respectively. cP<0.01 vs CTRL group, fP<0.01 vs E2-treated group.
