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. 2010 Sep 13;7(6):459–470. doi: 10.1038/cmi.2010.43

Figure 2.

Figure 2

Trif−/− mice upregulate Th17 responses and downregulate Treg responses. Mice were infected i.n. with live Aspergillus conidia. (a, b) Cytokine gene expression in the lung at different dpi (RT-PCR). (c) Cytokine gene expression in CD4+ T cells from thoracic lymph nodes at 7 dpi (RT-PCR). (d) Absolute numbers of γδ TCR+ in the lung; (e) Il23r expression on purified γδ TCR+ cells by real-time RT-PCR; (f) number of γδ TCR+ cells producing IL-17A or IFN-γ by ELISPOT assay. (g) Percentage of CD4+CD25+ T cells in the lung at different dpi and Foxp3, Icos and Ctla4 gene expression by real-time RT-PCR. Data are pooled from three experiments. *P<0.05, **P<0.01, ***P<0.001, Trif−/− versus WT mice. dpi, days post-infection; IFN, interferon; i.n., intranasally; RT-PCR, PCR with reverse transcription; Th17, T helper 17; WT, wild-type.