Table 1. Sequences of PCR primers.
| Name | Sequence (5′ to 3′)a | |
|---|---|---|
| F1 | ATGGGAACCCCAAAGCCACG | For cloning of IRF 3 and IRF3-CL ORF |
| R1 | TTATTGGTTGAGG TGGTGGGGAAC | |
| LCH-17F | GCTCAAGCTTCGAATTCTGGAACCC CAA AGCCACGGATC | Fusion of IRF3-CL with EGFP |
| LCH-18R | GCCCGCGGTACCGTCGACTTATTGGTTGAGGTGGTGGGGAAC | |
| LCH-17F | GCTCAAGCTTCGAATTCTGGAACCCCA AAGCCACGGATC | Fusion of IRF3 with DsRed |
| LCH-20R | GCCCGCGGTACCGTCGACTCAGGTCTC CCCAGGGCCCT | |
| LCH-25F | TTACCGGAACCCCAAAGCCACGGATC | Fusion of IRF3-CL with Myc tag |
| LCH-26R | TGACTTATTGGTTGAGGTGGTGGGGAAC | |
| LCH-25F | TTACCGGAACCCCAAAGCCACGGATC | Fusion of IRF3 with Myc tag |
| LCH-28R | TGACTCAGGTCTCCCCAGGGCCCT | |
| LCH-29F | CGCGAAGGAACCCCAAAGCCACGGATC | Fusion of IRF3-CL with Flag tag |
| LCH-30R | CGATTATTGGTTGAGGTGGTGGGGAAC | |
| LCH-29F | CGCGAAGGAACCCCAAAGCCACGGATC | Fusion of IRF3-CL with Flag tag |
| LCH-32R | CGATCAGGTCTCCCCAGGGCCCT |
Abbreviations: EGFP, enhanced green fluorescent protein; IRF3, interferon regulatory factor-3; ORF, open reading frame.
a
The sequence underlined at the 5′ end of each primer is the additional non-specific sequence used for cloning.