Figure 4.

Effects of β-arrestin-2′ downregulation on the anti-inflammation and redistribution of LPS-stimulated TLR4/CD14 complex stimulated by β₂-AR. (A) Effect of β-arrestin-2 small interfering RNA (siRNA) for 72 h on the expression of the β-arrestin-2 protein. Data are presented as mean±SEM. ^bP<0.05 vs control or scramble. GAPDH was used as an internal loading control. (B) After transfection with siRNA designed against β-arrestin-2 for 72 h, confocal analysis of LPS-stimulated TLR4/CD14 complex in THP-1 cells (a); Confocal analysis of redistribution of LPS-stimulated TLR4/CD14 complex in THP-1 cells under stimulation with β₂-AR (b). (C) After transfection with siRNA designed aga inst β-arrestin-2 for 72 h, THP-1 cells were stimulated with LPS for 24 h in the presence or absence of fenoterol, anti-inflammatory effects stimulated by β₂-AR was abolished when β-arrestin-2 was knocked down. ^bP<0.05 vs LPS 0.1 μg/mL.