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. 2013 May 27;34(8):1093–1100. doi: 10.1038/aps.2013.44

Figure 8.

Figure 8

(A) The levels of intracellular ATP decreased after treatment with fluopsin C in a time-dependent manner. The ATP level in MCF-7 cells without fluopsin C treatment was set as 100%. bP<0.05, cP<0.01 vs the control (0 h). (B) Fluopsin C treatment led to the accumulation of intracellular ROS in MCF-7 cells, as assessed with flow cytometric analysis. The cells were treated with 2 μmol/L fluopsin C for 0 h to 9 h. ROS accumulation was quantified by measuring DCF-derived fluorescence with flow cytometry after incubating the cells with DCFH-DA for 30 min. (C) Fluopsin C induced the loss of Δψm, as shown by flow cytometry. bP<0.05, cP<0.01 vs the control (0 h). The Δψm in MCF-7 cells without fluopsin C treatment was set as 100%.