Figure 5.

TLR4^−/−-derived DCs remained immature after TLR4^+/+→BALB/c or TLR4^−/−→BALB/c transplantations. Phenotypic analysis by flow cytometry of in vivo experiments: upregulation of costimulatory markers on splenic DCs from TLR4^+/+→BALB/c mice after BMT. Expression of CD80 and CD86 in DCs obtained from spleen cells of TLR4^+/+→BALB/c or TLR4^−/−→BALB/C mice 21 days after BMT. Gray represents an isotype control. The green line represents TLR4^+/+→BALB/c cells and the blue line represents TLR4^−/−→BALB/c cells. (a) Cells were stained with FITC-anti-MHC-II antibodies, PE-anti-CD80 or CD86 antibodies, PerCP-anti-CD11c antibodies and analyzed by flow cytometry. The analysis was performed on living cells gated by the CD11c^hiMHC-II^hi population. Both CD80 and CD86 were expressed in a lower amount by TLR4^−/− mice cells (b and c). Two independent experiments, with two mice for each, were performed. BMT, bone marrow transplantation; DC, dendritic cell; TLR4, Toll-like receptor 4.