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Emodin inhibited 11β-HSD1 activity in 3T3-L1 adipocytes after 1 h (A) or 24 h (B) of treatment. Differentiated 3T3-L1 adipocytes were incubated with the indicated concentrations of emodin or 0.1% DMSO for 1 h or 24 h. 11β-HSD1 enzyme activity was determined by scintillation proximity assay. Each point represents the mean±SEM. n=3.
