Figure 5.

Effect of hematoxylin on the proliferation and the phosphorylation of IGF1R and downstream signaling pathways in HL-60 cells. (A) Growth inhibition of HL-60 cells by hematoxylin. Cells were treated with the indicated concentrations of hematoxylin for 72 h. Cell viability was determined by sulforhodamine B assay. The tests were repeated three times independently. (B) Hematoxlyin inhibits the phosphorylation of IGF1R and the activation of downstream signaling pathways in HL-60 cells. Cells were cultured in the presence of different doses of hematoxylin for 24 h and harvested. Whole cell lysates were assayed for different proteins by immunoblotting. A representative anti-GAPDH immunoblot is shown as a loading control. Data shown were mean±SD from three independent experiments.