Figure 6.

Hematoxylin induces apoptosis in HL-60 cells via both intrinsic and extrinsic apoptosis pathways. (A) DAPI staining assay. DAPI-stained nuclei of cells untreated or treated with 20 μmol/L hematoxylin were observed using a microcopy (×200). (B) PI staining for flow cytometry. Cells were treated with hematoxylin for 24 h at indicated concentrations and then analyzed using FACS after they were fixed by 70% ethanol and stained with PI. Sub G₀/G₁ DNA content of cells were indicated. (C) Hematoxylin modulates the expression of apoptosis-related proteins. After treatment with or without desired concentration of hematoxylin for 24 h, cells were collected and assayed by Western blotting analysis.