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. 2011 Jun 13;32(7):939–946. doi: 10.1038/aps.2011.28

Figure 4.

Figure 4

dsP21-322 transfection-induced apoptosis in HCC cells. (A) SMMC-7721 or MHCCLM3 cells were double-stained with annexin V and PI 72 h after treatment with dsP21-322 (50 nmol/L), dsControl (50 nmol/L), or mock transfection and analyzed by flow cytometry. Gate settings distinguished between live (bottom left), necrotic (top left), early apoptotic (bottom right), and late apoptotic (top right) cells. (B) Changes in the levels of Bcl-xL anti-apoptotic gene products in MHCCLM3 cells 72 h after treatment with 50 nmol/L dsP21-322. (C) Effects of dsP21-322 transfection-activated p21 levels on cleavage of apoptosis-related proteins caspase-3, caspase-9, and poly (ADP-ribose) polymerase (PARP). Beta-actin levels served to normalize loading. The data shown are from a representative experiment repeated three times with similar results.