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Effects of p21 knockdown on cell proliferation and apoptosis of MHCCLM3 cells. Either 48 or 72 h after cells were treated with siP21 (50 nmol/L), dsControl (50 nmol/L), or mock transfection, messenger RNA levels (A) were determined by qPCR analysis, and Western blot (B) was used to analyze p21 knockdown efficiency. Cell proliferation (C) and apoptosis (D) were assessed as described previously. bP<0.05 vs mock transfections.
