Table 3. Effects of diabetes on Rho123 distribution in cerebral cortex, hippocampus, liver, intestinal mucosa and kidney of experimental rats.
| Control | Diabetes | Diabetes+insulin | |
|---|---|---|---|
| 5-week | |||
| Plasma (ng/mL) | 44.4±5.5 | 30.6±1.8c | 45.6±4.0f |
| Cerebral cortex (ng/g) | 4.28±0.14 | 5.15±0.43b | 3.80±0.26f |
| Kp (cerebral cortex, mL/g) | 0.09±0.01 | 0.17±0.01c | 0.09±0.01f |
| Hippocampus (ng/g) | 4.62±0.63 | 6.26±1.32 | 7.11±2.59 |
| Kp (hippocampus, mL/g) | 0.11±0.02 | 0.21±0.02c | 0.14±0.06 |
| Liver (ng/g) | 52.5±17.3 | 187.1±47.5c | 87.6±45.4f |
| Kp (liver, mL/g) | 1.16±0.26 | 6.17±1.41c | 1.87±0.87f |
| Intestinal mucosa (ng/g) | 451 811±68 825 | 527 034±14 232 | 275 987±76 654b |
| Kp (intestinal mucosa, mL/g) | 12 653±3375 | 17 345±1224 | 6308±1819bf |
| Kidney (ng/g) | 784.2±237.7 | 976.9±114.9 | 594.4±195.3e |
| Kp (kidney, mL/g) | 17.7±4.7 | 32.7±5.3c | 13.3±5.1f |
| 8-week | |||
| Plasma (ng/mL) | 38.4±4.8 | 25.3±1.7c | 40.7±0.7f |
| Cerebral cortex (ng/g) | 4.04±0.34 | 3.19±0.26c | 3.45±0.42 |
| Kp (cerebral cortex, mL/g) | 0.10±0.01 | 0.13±0.02 | 0.09±0.01f |
| Hippocampus (ng/g) | 3.70±0.20 | 3.69±0.47 | 4.37±0.64 |
| Kp (hippocampus, mL/g) | 0.10±0.02 | 0.15±0.02c | 0.11±0.02f |
| Liver (ng/g) | 69.4±10.2 | 66.6± 6.2 | 120.9±34.1be |
| Kp (liver, ml/g) | 1.77±0.11 | 2.70±0.27 | 2.98±0.85b |
| Intestinal mucosa (ng/g) | 101 135±23 658 | 77 296±8577 | 210 123±55 354bf |
| Kp (intestinal mucosa, mL/g) | 2777±366 | 3087±331 | 5170±1321 |
| Kidney (ng/g) | 422.5±103.2 | 149.3±34.1c | 578.0±139.9bf |
| Kp (kidney, mL/g) | 17.9±3.4 | 5.9±1.4c | 14.2±3.2f |
The insulin-treated rats (Diabetes+insulin) were subcutaneouly administered insulin at 10 U·kg−1·d−1 twice a day for consecutive 5 and 8 weeks. The STZ-induced diabetic rats (Diabetes) and age-matched normal control rats (Control) only received vehicle. The concentrations of Rho123 in plasma and tissues were measured at 45 min followed administration of Rho123 (0.2 mg/kg, iv). Each data represents the mean±SD of five rats. Intestinal mucosa was represented by ng/g protein, while brain, liver and kidney were represented by ng/g tissue. Kp represents the tissue-to-plasma concentration ratio. bP<0.05, cP<0.01 vs control group; eP<0.05, fP<0.01 vs diabetic group.