Table 1. Differentially expressed proteins in the kidney of mouse treated with ASB identified by TOF/TOF MS.
| Spot | Protein | Gene | Accession (UnifrotKB) | Functional classification | MW* (kDa) | pI theoretical | pI estimated | Mascot score | Sequence coverage | ASB effect | Fold change |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Methylmalonyl-Coenzyme A mutase (MUT) | Mut | Q3UJU1 | Amino acid degradation; propanoate metabolism | 82791.6 | 6.45 | 5.95 | 158 | 25% | ↑ | 4.9 |
| 5 | Peroxiredoxin-6 (PRDX6) | Prdx6 | O08709 | Response to ROS | 24871 | 5.7 | 5.7 | 351 | 40% | ↑ | 4.6 |
| 6 | Nucleoside diphosphate kinase B (NDK B) | Nme2 | Q01768 | Nucleotide metabolism | 17351.9 | 6.97 | 7.48 | 184 | 59% | ↑ | 1.9 |
| 2 | Heat shock cognate 71 kDa protein (HSC70) | Hspa8, Hsc70 | P63017 | Stress response | 68736.2 | 5.37 | 5.4 | 225 | 21% | ↓ | 2.3 |
| 3 | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subuint 10, mitochondrial (NDUFA10) | Ndufa10 | Q99LC3 | Oxidative phosphorylation | 40577.7 | 7.63 | 6.1 | 72 | 23% | ↓ | 1.3 |
| 7 | Nucleoside diphosphate kinase B (NDK B) | Nme2 | Q01768 | Nucleotide metabolism | 17351.9 | 6.97 | 6.97 | 252 | 46% | ↓ | 6.2 |
| 4 | Nucleoside diphosphate-linked moiety X motif 19, mitochondrial (Nudix motif19, RP2p) | Nudt19, D7Rp2e | P11930 | Fatty acid oxidation | 40294.4 | 6.22 | 6.18 | 261 | 16% | shift | – |
*Molecular weights estimated from the experiment data in this study are all similar to the theoretic one.