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PF markedly inhibits cell activation. Purified T cells were cultured with or without anti-CD3 and anti-CD28 in the presence or absence of 25% PF. FACS analysis for the expression of CD69 (a) and CD25 (b) on CD4+ and CD8+ T cells was performed. Isotype controls are shown as grey areas, and expression of activation markers are depicted as solid lines. Data are representative of five experiments with similar results. PF, pleural fluid.
