Figure 3.

Expression and activity of caspases in human T cells after emodin treatment. (A) Pro- and cleaved caspase-9, -4, and -3 of human T cells after emodin treatment with different doses were detected by Western blotting. (B) Pro- and cleaved caspase-8 were detected by Western blotting. (C), (D) and (E) The results of Western blotting were analyzed by Quantity One software. (F), (G), and (H) Activity of caspase-9, -4, and -3 were detected by caspase activity assay. The results are given as mean±SD from three experiments. ^bP<0.05, ^cP<0.01 vs control.