Figure 3.

Protective effect of CYP450 inhibitors on oxidative stress induced by tetrandrine. (A) Effects of CYP450 inhibitors on ROS production induced by tetrandrine. ROS were detected using the fluorescent probe DCFH-DA. Rat hepatocytes were incubated with 25 μmol/L Tet and the indicated inhibitors for 1 h. The concentration of each inhibitor is listed in Table 1. (B) Effect of SKF on the depletion of GSH induced by tetrandrine (10 and 25 μmol/L). GSH content was measured in rat primary hepatocytes at time points up to 8 h in either the presence or absence of SKF (10 μmol/L). All of the results are presented as the mean±SD. n=4. ^cP<0.01 vs 'Tet 25 μmol/L' group.