Figure 1.

The concentration-response curves of SKF83959 and DOV21947 on SERT, NET, and DAT. The uptake assays were performed as described in the Methods section. Various concentrations of SKF83959 or DOV21947 were incubated with [³H]-radiolabeled ligand for 10 min. The final concentration of [³H]-serotonin was 2.4 nmol/L, [³H]-norepinephrine was 1.4 nmol/L and [³H]-dopamine was 1.6 nmol/L. The nonspecific uptake activity was determined using Na⁺-free HBSS solution and the specific uptake activity was normalized as 100%. Each experiment was replicated at least three times. The data were expressed as the mean±SEM.