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. 2014 May;143(5):645–656. doi: 10.1085/jgp.201311036

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© 2014 Pless et al.

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Figure 4. — Replacing the S2 aromatic with Leu has drastic functional consequences in DII–DIV. (A) Chemical structure of Phe and Leu. (B) Sample traces for currents recorded from WT or mutants that replaced the S2 aromatic with Leu (DI, Tyr168Leu; DII, Phe621Leu; DIII, Phe1076Leu; DIV, Phe1396Leu). Bars: horizontal, 5 ms; vertical, 500 nA. Voltage steps were from −40 to +20 mV in 10-mV increments. (C and D) G-V (C) and SSI curves (D) for WT and mutants in which the S2 aromatic was replaced by Leu; the inset in D shows a bar graph representing the average time constants for fast inactivation (τ) for a depolarizing voltage step to −15 mV for WT and the Leu mutants in DI–DIV. *, statistical difference to WT values in an unpaired t test (P < 0.01).