Table 1.
Construct | Location | G-V | SSI | n | ||
V1/2 | dx | V1/2 | dx | |||
WT | n/a | −26.3 ± 0.5 | 3.0 ± 0.1 | −52.9 ± 0.5 | 5.7 ± 0.1 | 17 |
Ser151Glu | DI S1 (ENC) | −32.7 ± 1.1a | 2.5 ± 0.2 | −53.2 ± 0.9 | 5.4 ± 0.1 | 6 |
Glu598Gln | DII S1 (ENC) | −17.4 ± 0.7a | 2.7 ± 0.2 | −50.6 ± 0.8 | 6.7 ± 0.4 | 5 |
Glu1051Gln | DIII S1 (ENC) | −24.8 ± 0.7 | 2.5 ± 0.2 | −46.1 ± 0.9a | 5.0 ± 0.2a | 6 |
Glu1373Gln | DIV S1 (ENC) | −27.3 ± 2.6 | 3.3 ± 0.2 | −40.7 ± 1.2a | 4.6 ± 0.1a | 6 |
Glu161TAG + Nha | DI S2 (ENC) | −14.8 ± 0.3a | 3.1 ± 0.1 | −50.0 ± 0.2a | 5.9 ± 0.1 | 3 |
Asn614Asp | DII S2 (ENC) | −32.0 ± 0.6a | 3.2 ± 0.1 | −57.2 ± 1.0a | 5.4 ± 0.3 | 5 |
Asp1069Asn | DIII S2 (ENC) | −27.3 ± 0.7 | 2.7 ± 0.2 | −50.7 ± 0.5 | 5.2 ± 0.2 | 5 |
Glu171TAG + Nha | DI S2 (INC) | −26.8 ± 0.7 | 3.0 ± 0.1 | −52.9 ± 0.9 | 5.1 ± 0.2a | 9 |
Glu624Gln | DII S2 (INC) | −22.7 ± 0.5a | 3.6 ± 0.1 | −51.6 ± 0.8 | 6.0 ± 0.8 | 6 |
Glu1079Gln | DIII S2 (INC) | −27.6 ± 1.0 | 3.0 ± 0.2 | −55.6 ± 1.0 | 5.4 ± 0.2 | 6 |
Glu1399TAG + Nha | DIV S2 (INC) | −24.7 ± 0.5 | 3.2 ± 0.1 | −46.2 ± 0.8a | 7.1 ± 0.2a | 6 |
Asp197Asn | DI S3 (INC) | −26.0 ± 0.6 | 3.4 ± 0.2 | −52.9 ± 0.8 | 7.1 ± 0.4a | 7 |
Asp646Asn | DII S3 (INC) | −22.5 ± 1.6 | 2.9 ± 0.2 | −55.7 ± 1.6 | 6.0 ± 0.8 | 9 |
Asp1101Asn | DIII S3 (INC) | −27.6 ± 0.7 | 3.3 ± 0.1 | −58.1 ± 0.7a | 6.5 ± 0.4 | 7 |
Asp1420Asn | DIV S3 (INC) | −25.8 ± 1.4 | 3.3 ± 0.2 | −46.2 ± 1.7a | 6.9 ± 0.3a | 7 |
Displayed are the values for the midpoints (V1/2) and the slope factors (dx) of the G-V relations and the SSI, as well as the number of experiments conducted (n).
Statistical difference to WT values in an unpaired t test (P < 0.01).