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. 2014 May;143(5):633–644. doi: 10.1085/jgp.201311112

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© 2014 Gao et al.

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Figure 5. — ¹O₂ mediates photochemical transformation of the mHCN2 channel. (A) Construction of the HCN2-SOG fusion channel. (B; top) Bright field and fluorescence images of a membrane patch expressing HCN2-SOG channels. (Bottom) Excitation and emission spectra of purified mini-SOG protein. (C) Timing of the laser pulse and the voltage protocol. (D) Current traces before (black; no. 3 in E) and after (blue; no. 7 in E) laser treatments. (E) τ_deactivation or percentage of I_inst versus episode number. Neither cAMP nor FITC-cAMP was applied. *, P < 0.05.