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. 2013 Jul 22;10(5):393–402. doi: 10.1038/cmi.2013.16

Figure 1.

Figure 1

In vivo infusion of apoptotic cells facilitates islets engraftment. (a) Splenocytes were exposed to UVB radiation for 10 min and incubated for 4 h in vitro. Treated (right panel) and untreated (left panel) cells were then stained with FITC-Annexin V and PI to detect apoptosis induction. Results are representative of three independent experiments. (b) Isolated pancreatic islets stained with DTZ (×100). (c) Graft survival of diabetic mice receiving transplantation with (gray solid line, MST=56.9±1.8 days) or without (black solid line, MST=7.6±0.8 days) donor apoptotic splenocytes pre-infusion. Graft survival of diabetic mice receiving live splenocyte pre-infusion is showed in gray dashed line (n=10, P<0.01). (d) Histological analysis of the transplanted islets. Tolerant recipients with apoptotic splenocytes pre-infusion had minor lymphocytes infiltration (right panel) compared to the rejecting recipients (left panel). (e) Blood glucose levels of the mice after STZ injection. The mice received apoptosis cells or PBS injection on day 7 and transplantation on day 14. Apoptotic splenocytes administrations facilitate the engraftment of islets and their function. DTZ, dithizone; MST, mean survival time; PBS, phosphate-buffered saline; PI, propidium iodide; STZ, streptozocin.