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. 2013 Jul 22;10(5):444–452. doi: 10.1038/cmi.2013.22

Figure 2.

Figure 2

PPD treatment and BCG infection induces Mcl-1 expression in BMM. (a) BMM were treated with PPD (5 µg/ml) for the indicated times, and the expression of Mcl-1, Notch1 and cleaved Notch1 (Val1744) were detected by western blot. (b) BMM were infected with BCG (MOI 1∶10) for the indicated times, and the expression of Mcl-1 and Notch1 were detected by western blot. (c, d) BMM were treated with PPD (c) or infected with BCG (d) for the indicated times, and the levels of mcl-1 mRNA were detected by qPCR. Cells without any stimulation was labeled as ‘unstimulated' and cells without BCG infection was labeled ‘uninf'. Cells The results represent two independent experiments carried out in triplicate. * indicates where statistical significance (P<0.05) was observed between the levels in uninfected cells and cells infected for 10 and 28 h, respectively. BCG, Bacillus Calmette-Guérin; BMM, bone marrow-derived macrophage; Mcl-1, myeloid cell leukemia sequence-1; MOI, multiplicity of infection; PPD, purified protein derivative; qPCR, quantitative real-time PCR.