Figure 5.

Effects of the transwell insert on the induction of (a, b) CCL2 and (c, d) CXCL8 release in coculture of eosinophils and bronchial epithelial cells upon NOD1 and NOD2 ligand stimulation. Eosinophils (5×10⁵ cells) and bronchial epithelial cells (8×10⁴ cells) were cultured together with (a, c) iE-DAP or (b, dD) MDP (10 µg/ml) for 24 h in the presence or absence of the transwell insert. Cell-free culture supernatant was collected and chemokine released into the supernatant was quantified using CBA. Results are expressed as arithmetic mean plus s.d. of three independent experiments. *P<0.05, **P<0.01, ***P<0.001 when compared between groups denoted by the horizontal lines. BE: BEAS-2B cells; Eos: eoisnophils. CBA, cytometric bead array; iE-DAP, γ-D-glutamyl-meso-diaminopimelic acid; MDP, muramyl dipeptide; NOD, nucleotide-binding oligomerization domain; s.d., standard deviation.